PDBe 1du0

X-ray diffraction
2Å resolution

ENGRAILED HOMEODOMAIN Q50A VARIANT DNA COMPLEX

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Segmentation polarity homeobox protein engrailed Chains: A, B
Molecule details ›
Chains: A, B
Length: 57 amino acids
Theoretical weight: 6.91 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P02836 (Residues: 456-512; Coverage: 10%)
Gene names: CG9015, en
Structure domains: Homeodomain-like
DNA (5'-D(*AP*TP*TP*AP*GP*GP*TP*AP*AP*TP*TP*AP*CP*AP*TP*GP*GP*CP*AP*AP*A)-3') Chain: D
Molecule details ›
Chain: D
Length: 21 nucleotides
Theoretical weight: 6.49 KDa
Source organism: Synthetic construct
Expression system: Not provided
DNA (5'-D(*TP*TP*TP*TP*GP*CP*CP*AP*TP*GP*TP*AP*AP*TP*TP*AP*CP*CP*TP*AP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.39 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 126.9Å b: 45.55Å c: 71.62Å
α: 90° β: 119.9° γ: 90°
R-values:
R R work R free
0.237 0.234 0.27
Expression systems:
  • Escherichia coli
  • Not provided