1dpx Citations

Crystallization, structure solution and refinement of hen egg-white lysozyme at pH 8.0 in the presence of MPD.

Acta Crystallogr. D Biol. Crystallogr. 56 952-8 (2000)
Cited: 10 times
EuropePMC logo PMID: 10944331


Hen egg-white lysozyme has been crystallized at slightly alkaline pH using 2-methyl-2,4-pentanediol (MPD) as the precipitant. The crystals are nearly isomorphous to crystals grown at acidic pH using sodium chloride as the precipitant. However, the growth kinetics differ markedly between the two conditions. The major reason for this is a molecule of MPD that binds tightly in between two lysozyme molecules and favors the growth of the crystals along the crystallographic c direction over growth perpendicular to it.

Articles - 1dpx mentioned but not cited (3)

  1. An RNA aptamer possessing a novel monovalent cation-mediated fold inhibits lysozyme catalysis by inhibiting the binding of long natural substrates. Padlan CS, Malashkevich VN, Almo SC, Levy M, Brenowitz M, Girvin ME. RNA 20 447-461 (2014)
  2. Fitmunk: improving protein structures by accurate, automatic modeling of side-chain conformations. Porebski PJ, Cymborowski M, Pasenkiewicz-Gierula M, Minor W. Acta Crystallogr D Struct Biol 72 266-280 (2016)
  3. Innovative High-Throughput SAXS Methodologies Based on Photonic Lab-on-a-Chip Sensors: Application to Macromolecular Studies. Rodríguez-Ruiz I, Radajewski D, Charton S, Phamvan N, Brennich M, Pernot P, Bonneté F, Teychené S. Sensors (Basel) 17 (2017)

Reviews citing this publication (1)

Articles citing this publication (6)

  1. Effect of alcohols on aqueous lysozyme-lysozyme interactions from static light-scattering measurements. Liu W, Bratko D, Prausnitz JM, Blanch HW. Biophys. Chem. 107 289-298 (2004)
  2. NMR spectroscopic detection of protein protons and longitudinal relaxation rates between 0.01 and 50 MHz. Bertini I, Gupta YK, Luchinat C, Parigi G, Schlörb C, Schwalbe H. Angew. Chem. Int. Ed. Engl. 44 2223-2225 (2005)
  3. Protecting role of cosolvents in protein denaturation by SDS: a structural study. Michaux C, Pouyez J, Wouters J, Privé GG. BMC Struct. Biol. 8 29 (2008)
  4. Comparative estimation of vibrational entropy changes in proteins through normal modes analysis. Carrington BJ, Mancera RL. J. Mol. Graph. Model. 23 167-174 (2004)
  5. How to avoid premature decay of your macromolecular crystal: a quick soak for long life. Kauffmann B, Weiss MS, Lamzin VS, Schmidt A. Structure 14 1099-1105 (2006)
  6. A novel transition-state analogue for lysozyme, 4-O-β-tri-N-acetylchitotriosyl moranoline, provided evidence supporting the covalent glycosyl-enzyme intermediate. Ogata M, Umemoto N, Ohnuma T, Numata T, Suzuki A, Usui T, Fukamizo T. J. Biol. Chem. 288 6072-6082 (2013)