Function and Biology

THE MOBIL FLAVIN OF 4-OH BENZOATE HYDROXYLASE: MOTION OF A PROSTHETIC GROUP REGULATES CATALYSIS

Source organism: Pseudomonas aeruginosa
Biochemical function: 4-hydroxybenzoate 3-monooxygenase activity
Biological process: oxidation-reduction process
Cellular component: not assigned

EC 1.14.13.2: 4-hydroxybenzoate 3-monooxygenase

Reaction catalysed:
4-hydroxybenzoate + NADPH + O(2) = protocatechuate + NADP(+) + H(2)O.
Comments:
  • Most enzymes from Pseudomonas are highly specific for NAD(P)H (cf. EC 1.14.13.33).
Systematic name:
4-hydroxybenzoate,NADPH:oxygen oxidoreductase (3-hydroxylating)
Alternative Name(s):
  • 4-hydroxybenzoate 3-hydroxylase
  • 4-hydroxybenzoate monooxygenase
  • 4-hydroxybenzoic hydroxylase
  • p-hydroxybenzoate hydrolyase
  • p-hydroxybenzoate hydroxylase
  • p-hydroxybenzoate-3-hydroxylase
  • p-hydroxybenzoic acid hydrolase
  • p-hydroxybenzoic acid hydroxylase
  • p-hydroxybenzoic hydroxylase
  • Para-hydroxybenzoate hydroxylase

Sequence family

Pfam Protein family (Pfam)
PF01494
Domain description: FAD binding domain
Occurring in:
  1. p-hydroxybenzoate hydroxylase
1 copy of Pfam domain PF01494 (FAD binding domain) in p-hydroxybenzoate hydroxylase in PDB 1doc.

InterPro InterPro annotations
IPR036188
Domain description: FAD/NAD(P)-binding domain superfamily
Occurring in:
  1. p-hydroxybenzoate hydroxylase
IPR012733
Domain description: 4-hydroxybenzoate 3-monooxygenase
Occurring in:
  1. p-hydroxybenzoate hydroxylase
IPR002938
Domain description: FAD-binding domain
Occurring in:
  1. p-hydroxybenzoate hydroxylase

Structure domains

CATH CATH domains
3.30.9.10
Class: Alpha Beta
Architecture: 2-Layer Sandwich
Topology: D-Amino Acid Oxidase; Chain A, domain 2
Homology: D-Amino Acid Oxidase, subunit A, domain 2
Occurring in:
  1. p-hydroxybenzoate hydroxylase
1 copy of CATH domain 3.30.9.10 (D-Amino Acid Oxidase; Chain A, domain 2) in p-hydroxybenzoate hydroxylase in PDB 1doc.
3.50.50.60
Class: Alpha Beta
Architecture: 3-Layer(bba) Sandwich
Topology: FAD/NAD(P)-binding domain
Homology: FAD/NAD(P)-binding domain
Occurring in:
  1. p-hydroxybenzoate hydroxylase
1 copy of CATH domain 3.50.50.60 (FAD/NAD(P)-binding domain) in p-hydroxybenzoate hydroxylase in PDB 1doc.
SCOP SCOP annotations
51913
Class: Alpha and beta proteins (a/b)
Fold: FAD/NAD(P)-binding domain
Superfamily: FAD/NAD(P)-binding domain
Occurring in:
  1. p-hydroxybenzoate hydroxylase
1 copy of SCOP domain 51913 (FAD-linked reductases, N-terminal domain) in p-hydroxybenzoate hydroxylase in PDB 1doc.
54378
Class: Alpha and beta proteins (a+b)
Fold: FAD-linked reductases, C-terminal domain
Superfamily: FAD-linked reductases, C-terminal domain
Occurring in:
  1. p-hydroxybenzoate hydroxylase
1 copy of SCOP domain 54378 (PHBH-like) in p-hydroxybenzoate hydroxylase in PDB 1doc.