PDBe 1do8

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF A CLOSED FORM OF HUMAN MITOCHONDRIAL NAD(P)+-DEPENDENT MALIC ENZYME

Released:
Source organism: Homo sapiens

Function and Biology Details

Reaction catalysed:
(1) (S)-malate + NAD(+) = pyruvate + CO(2) + NADH. (2) Oxaloacetate = pyruvate + CO(2). 
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD-dependent malic enzyme, mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 564 amino acids
Theoretical weight: 63.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P23368 (Residues: 21-584; Coverage: 97%)
Gene name: ME2
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2
Unit cell:
a: 229Å b: 118.7Å c: 113Å
α: 90° β: 109.6° γ: 90°
R-values:
R R work R free
0.214 0.204 0.263
Expression system: Escherichia coli