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THE X-RAY STRUCTURE OF THE DNASE I-D(GGTATACC)2 COMPLEX AT 2.3 ANGSTROMS RESOLUTION

X-ray diffraction
2.3Å resolution
PDB entry 1dnk coloured by chain, front view.
PDB entry 1dnk coloured by chain, side view.
PDB entry 1dnk coloured by chain, top view.
Source organism: Bos taurus
Assembly composition:
hetero trimer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Deoxyribonuclease-1 and DNA
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Multimeric state: hetero trimer
Accessible surface area: 12053.89 Å2
Buried surface area: 2618.73 Å2
Dissociation area: 734.99 Å2
Dissociation energy (ΔGdiss): 8.47 kcal/mol
Dissociation entropy (TΔSdiss): 9.7 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-113697

Macromolecules

Deoxyribonuclease-1
Chain: A
Length: 260 amino acids
Theoretical weight: 29.09 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00639 (Residues: 23-282; Coverage: 100%)
Gene names: DNASE1, DNL1
Pfam: Endonuclease/Exonuclease/phosphatase family
InterPro:
CATH: Endonuclease/exonuclease/phosphatase
SCOP: DNase I-like
Deoxyribonuclease-1 in PDB entry 1dnk, assembly 1, front view.
Deoxyribonuclease-1 in PDB entry 1dnk, assembly 1, side view.
Deoxyribonuclease-1 in PDB entry 1dnk, assembly 1, top view.
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DNA chain B
Name: DNA (5'-D(*GP*GP*TP*AP*TP*AP*C)-3')
Representative chains: B
Source organism: Bos taurus [9913]
Expression system: Not provided
Length: 7 nucleotides
Theoretical weight: 2.14 KDa
DNA (5'-D(*GP*GP*TP*AP*TP*AP*C)-3') in PDB entry 1dnk, assembly 1, front view.
DNA (5'-D(*GP*GP*TP*AP*TP*AP*C)-3') in PDB entry 1dnk, assembly 1, side view.
DNA (5'-D(*GP*GP*TP*AP*TP*AP*C)-3') in PDB entry 1dnk, assembly 1, top view.
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DNA chain C
Name: DNA (5'-D(*GP*GP*TP*AP*TP*AP*CP*C)-3')
Representative chains: C
Source organism: Bos taurus [9913]
Expression system: Not provided
Length: 8 nucleotides
Theoretical weight: 2.43 KDa
DNA (5'-D(*GP*GP*TP*AP*TP*AP*CP*C)-3') in PDB entry 1dnk, assembly 1, front view.
DNA (5'-D(*GP*GP*TP*AP*TP*AP*CP*C)-3') in PDB entry 1dnk, assembly 1, side view.
DNA (5'-D(*GP*GP*TP*AP*TP*AP*CP*C)-3') in PDB entry 1dnk, assembly 1, top view.
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