PDBe 1dmu

X-ray diffraction
2.2Å resolution

Crystal structure of the restriction endonuclease BglI (e.c. bound to its dna recognition sequence


Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Type-2 restriction enzyme BglI Chain: A
Molecule details ›
Chain: A
Length: 299 amino acids
Theoretical weight: 34.05 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
  • Canonical: O68557 (Residues: 1-299; Coverage: 100%)
Gene name: bglIR
Sequence domains: Restriction endonuclease BglI
Structure domains: ECO RV Endonuclease; Chain A
Molecule details ›
Chain: F
Length: 17 nucleotides
Theoretical weight: 5.21 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: C2221
Unit cell:
a: 78.48Å b: 81.6Å c: 117.06Å
α: 90° β: 90° γ: 90°
R R work R free
0.185 0.177 0.239
Expression systems:
  • Escherichia coli
  • Not provided