PDBe 1dl2

X-ray diffraction
1.54Å resolution

CRYSTAL STRUCTURE OF CLASS I ALPHA-1,2-MANNOSIDASE FROM SACCHAROMYCES CEREVISIAE AT 1.54 ANGSTROM RESOLUTION

Released:

Function and Biology Details

Reaction catalysed:
Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man(9)(GlcNAc)(2). 
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase Chain: A
Molecule details ›
Chain: A
Length: 511 amino acids
Theoretical weight: 58.77 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P32906 (Residues: 34-549; Coverage: 93%)
Gene names: J2110, MNS1, YJR131W
Structure domains: Glycosyltransferase

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P3121
Unit cell:
a: 88.397Å b: 88.397Å c: 153.22Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.209 0.227
Expression system: Komagataella pastoris