PDBe 1dki

X-ray diffraction
1.6Å resolution

CRYSTAL STRUCTURE OF THE ZYMOGEN FORM OF STREPTOCOCCAL PYROGENIC EXOTOXIN B ACTIVE SITE (C47S) MUTANT

Released:

Function and Biology Details

Reaction catalysed:
Preferential cleavage with hydrophobic residues at P2, P1 and P1'. 
Biochemical function:
Biological process:
Cellular component:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptopain Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 371 amino acids
Theoretical weight: 40.32 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C0J0 (Residues: 28-398; Coverage: 100%)
Gene name: speB
Sequence domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 46.664Å b: 116.556Å c: 144.726Å
α: 90° β: 94.17° γ: 90°
R-values:
R R work R free
0.219 0.218 0.245
Expression system: Escherichia coli