PDBe 1dkg

X-ray diffraction
2.8Å resolution

CRYSTAL STRUCTURE OF THE NUCLEOTIDE EXCHANGE FACTOR GRPE BOUND TO THE ATPASE DOMAIN OF THE MOLECULAR CHAPERONE DNAK

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chaperone protein DnaK Chain: D
Molecule details ›
Chain: D
Length: 383 amino acids
Theoretical weight: 41.57 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6Y8 (Residues: 1-383; Coverage: 60%)
Gene names: JW0013, b0014, dnaK, groP, grpF, seg
Structure domains:
Protein GrpE Chains: A, B
Molecule details ›
Chains: A, B
Length: 197 amino acids
Theoretical weight: 21.88 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P09372 (Residues: 1-197; Coverage: 100%)
Gene names: JW2594, b2614, grpE
Sequence domains: GrpE
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P41
Unit cell:
a: 149.43Å b: 149.43Å c: 49.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.223 0.317
Expression system: Escherichia coli