PDBe 1dj0

X-ray diffraction
1.5Å resolution

THE CRYSTAL STRUCTURE OF E. COLI PSEUDOURIDINE SYNTHASE I AT 1.5 ANGSTROM RESOLUTION

Released:

Function and Biology Details

Reaction catalysed:
tRNA uridine(38-40) = tRNA pseudouridine(38-40). 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA pseudouridine synthase A Chains: A, B
Molecule details ›
Chains: A, B
Length: 264 amino acids
Theoretical weight: 29.72 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P07649 (Residues: 7-270; Coverage: 98%)
Gene names: JW2315, asuC, b2318, hisT, leuK, truA
Sequence domains: tRNA pseudouridine synthase
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200, SSRL BEAMLINE BL7-1
Spacegroup: P21
Unit cell:
a: 68.08Å b: 37.4Å c: 103.48Å
α: 90° β: 97.68° γ: 90°
R-values:
R R work R free
0.162 0.167 0.221
Expression system: Escherichia coli