PDBe 1dio

X-ray diffraction
2.2Å resolution

DIOL DEHYDRATASE-CYANOCOBALAMIN COMPLEX FROM KLEBSIELLA OXYTOCA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Diol dehydrase alpha subunit Chains: A, L
Molecule details ›
Chains: A, L
Length: 554 amino acids
Theoretical weight: 60.41 KDa
Source organism: Klebsiella oxytoca
Expression system: Escherichia coli
UniProt:
  • Canonical: Q59470 (Residues: 1-554; Coverage: 100%)
Gene names: AB185_12495, CIG58_07275, SAMEA2273575_05741, pddA, pduC
Sequence domains: Dehydratase large subunit
Structure domains: TIM Barrel
Diol dehydrase beta subunit Chains: B, E
Molecule details ›
Chains: B, E
Length: 224 amino acids
Theoretical weight: 24.14 KDa
Source organism: Klebsiella oxytoca
Expression system: Escherichia coli
UniProt:
  • Canonical: Q59471 (Residues: 1-224; Coverage: 100%)
Gene name: pddB
Sequence domains: Dehydratase medium subunit
Structure domains: B12-dependent dehydatase associated subunit
Propanediol dehydratase small subunit Chains: G, M
Molecule details ›
Chains: G, M
Length: 173 amino acids
Theoretical weight: 19.2 KDa
Source organism: Klebsiella oxytoca
Expression system: Escherichia coli
UniProt:
  • Canonical: Q59472 (Residues: 1-173; Coverage: 100%)
Gene names: AB185_12485, KOSB73_30026, SAMEA2273639_01293, SAMEA2273697_01477, pddC, pduE, pduE_1
Sequence domains: Dehydratase small subunit

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 76.2Å b: 122.3Å c: 209.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.187 0.236
Expression system: Escherichia coli