PDBe 1dgs

X-ray diffraction
2.9Å resolution

CRYSTAL STRUCTURE OF NAD+-DEPENDENT DNA LIGASE FROM T. FILIFORMIS

Released:

Function and Biology Details

Reaction catalysed:
NAD(+) + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho- (deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + beta- nicotinamide D-nucleotide. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 667 amino acids
Theoretical weight: 76 KDa
Source organism: Thermus filiformis
UniProt:
  • Canonical: Q9ZHI0 (Residues: 1-232, 233-670; Coverage: 99%)
Gene name: ligA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P21
Unit cell:
a: 89.21Å b: 117.33Å c: 97.48Å
α: 90° β: 115.09° γ: 90°
R-values:
R R work R free
0.228 0.228 0.298