PDBe 1dan

X-ray diffraction
2Å resolution

Complex of active site inhibited human blood coagulation factor VIIA with human recombinant soluble tissue factor

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Tissue factor Chain: T
Molecule details ›
Chain: T
Length: 80 amino acids
Theoretical weight: 9.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P13726 (Residues: 37-116; Coverage: 30%)
Gene name: F3
Structure domains: Immunoglobulins
Tissue factor Chain: U
Molecule details ›
Chain: U
Length: 121 amino acids
Theoretical weight: 13.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P13726 (Residues: 122-242; Coverage: 46%)
Gene name: F3
Structure domains: Immunoglobulins
Factor VII heavy chain Chain: H
Molecule details ›
Chain: H
Length: 254 amino acids
Theoretical weight: 28.1 KDa
Source organism: Homo sapiens
Expression system: Mesocricetus auratus
UniProt:
  • Canonical: P08709 (Residues: 213-466; Coverage: 57%)
Gene name: F7
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Factor VII light chain Chain: L
Molecule details ›
Chain: L
Length: 152 amino acids
Theoretical weight: 17.49 KDa
Source organism: Homo sapiens
Expression system: Mesocricetus auratus
UniProt:
  • Canonical: P08709 (Residues: 61-212; Coverage: 34%)
Gene name: F7
Sequence domains:

Ligands and Environments


1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.5
Spacegroup: P212121
Unit cell:
a: 70.65Å b: 82.55Å c: 126.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.187 0.218
Expression systems:
  • Escherichia coli
  • Mesocricetus auratus