PDBe 1d8h

X-ray diffraction
2Å resolution

X-RAY CRYSTAL STRUCTURE OF YEAST RNA TRIPHOSPHATASE IN COMPLEX WITH SULFATE AND MANGANESE IONS.

Released:

Function and Biology Details

Reaction catalysed:
A 5'-phosphopolynucleotide + H(2)O = a polynucleotide + phosphate. 
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
mRNA-capping enzyme subunit beta Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 311 amino acids
Theoretical weight: 35.69 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O13297 (Residues: 241-549; Coverage: 56%)
Gene names: CET1, P1433, YPL228W
Sequence domains: mRNA capping enzyme, beta chain
Structure domains: mRNA Triphosphatase Cet1; Chain A

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2
Unit cell:
a: 116.436Å b: 118.723Å c: 85.138Å
α: 90° β: 108.06° γ: 90°
R-values:
R R work R free
0.229 0.229 0.31
Expression system: Escherichia coli BL21(DE3)