Function and Biology

MALATE SYNTHASE G COMPLEXED WITH MAGNESIUM AND GLYOXYLATE

Source organism: Escherichia coli
Biochemical function: transferase activity
Biological process: glyoxylate cycle
Cellular component: cytosol

EC 2.3.3.9: Malate synthase

Reaction catalysed:
Acetyl-CoA + H(2)O + glyoxylate = (S)-malate + CoA.
Comments:
  • The enzyme catalyzes the irreversible condensation of acetyl-CoA with glyoxylate to form (S)-malate.
  • Among other functions, the enzyme participates in the glyoxylate cycle, a modified version of the TCA cycle that bypasses steps that lead to a loss of CO(2).
  • Formerly EC 4.1.3.2.
Systematic name:
Acetyl-CoA:glyoxylate C-acetyltransferase ((S)-malate-forming)
Alternative Name(s):
  • L-malate glyoxylate-lyase (CoA-acetylating)
  • Glyoxylate transacetylase
  • Glyoxylate transacetase
  • Glyoxylic transacetase
  • Malate condensing enzyme
  • Malate synthetase
  • Malic synthetase
  • Malic-condensing enzyme
  • Acetyl-CoA:glyoxylate C-acetyltransferase (thioester-hydrolyzing, carboxymethyl-forming)

Sequence family

Pfam Protein family (Pfam)
PF01274
Domain description: Malate synthase
Occurring in:
  1. Malate synthase G
1 copy of Pfam domain PF01274 (Malate synthase) in Malate synthase G in PDB 1d8c.

InterPro InterPro annotations
IPR006253
Domain description: Malate synthase G
Occurring in:
  1. Malate synthase G
IPR001465
Domain description: Malate synthase
Occurring in:
  1. Malate synthase G
IPR011076
Domain description: Malate synthase-like superfamily
Occurring in:
  1. Malate synthase G

Structure domains

CATH CATH domains
3.20.20.360
Class: Alpha Beta
Architecture: Alpha-Beta Barrel
Topology: TIM Barrel
Homology: Malate synthase, domain 3
Occurring in:
  1. Malate synthase G
1 copy of CATH domain 3.20.20.360 (TIM Barrel) in Malate synthase G in PDB 1d8c.
2.170.170.11
Class: Mainly Beta
Architecture: Beta Complex
Topology: Malate synthase G - maily-beta sub-domain
Homology: Malate synthase G - maily-beta sub-domain
Occurring in:
  1. Malate synthase G
1 copy of CATH domain 2.170.170.11 (Malate synthase G - maily-beta sub-domain) in Malate synthase G in PDB 1d8c.
1.10.1860.10
Class: Mainly Alpha
Architecture: Orthogonal Bundle
Topology: N-terminal alpha-helical clasp
Homology: N-terminal alpha-helical clasp
Occurring in:
  1. Malate synthase G
1 copy of CATH domain 1.10.1860.10 (N-terminal alpha-helical clasp) in Malate synthase G in PDB 1d8c.
1.20.1220.12
Class: Mainly Alpha
Architecture: Up-down Bundle
Topology: Malate Synthase G; Chain: A; Domain 4
Homology: Malate Synthase G; Chain: A; Domain 4
Occurring in:
  1. Malate synthase G
1 copy of CATH domain 1.20.1220.12 (Malate Synthase G; Chain: A; Domain 4) in Malate synthase G in PDB 1d8c.
SCOP SCOP annotation
51646
Class: Alpha and beta proteins (a/b)
Fold: TIM beta/alpha-barrel
Superfamily: Malate synthase G
Occurring in:
  1. Malate synthase G
1 copy of SCOP domain 51646 (Malate synthase G) in Malate synthase G in PDB 1d8c.