› 1d6j

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF ADENOSINE 5'-PHOSPHOSULFATE (APS) KINASE FROM PENICILLIUM CHRYSOGENUM

Released:
DOI: 10.2210/pdb1d6j/pdb
PDB entry 1d6j coloured by chain, front view.
PDB entry 1d6j coloured by chain, side view.
PDB entry 1d6j coloured by chain, top view.
Source organism: Penicillium chrysogenum
Primary publication:
Crystal structure of adenosine 5'-phosphosulfate kinase from Penicillium chrysogenum.
MacRae IJ, Segel IH, Fisher AJ
Biochemistry 39 1613-21 (2000)
PMID: 10677210

Function and Biology Details

Reaction catalysed: 
ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171380 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylyl-sulfate kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 211 amino acids
Theoretical weight: 23.8 KDa
Source organism: Penicillium chrysogenum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12657 (Residues: 1-211; Coverage: 100%)
Sequence domains: Adenylylsulphate kinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand TLA 1 x TLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: C2221
Unit cell:
a: 78.862Å b: 83.484Å c: 141.951Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.207 0.249
Expression system: Escherichia coli

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Citations

3 review citations

Sulfotransferases and sulfatases in mycobacteria.
Mougous et al. (2002)
2 more

1 mention without citation

Discrimination between biological interfaces and crystal-packing contacts.
Tsuchiya et al. (2008)