PDBe 1d6i

X-ray diffraction
2Å resolution

CHALCONE SYNTHASE (H303Q MUTANT)

Released:

Function and Biology Details

Reaction catalysed:
3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO(2). 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chalcone synthase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 388 amino acids
Theoretical weight: 42.65 KDa
Source organism: Medicago sativa
Expression system: Escherichia coli
UniProt:
  • Canonical: P30074 (Residues: 2-389; Coverage: 100%)
Gene name: CHS2
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: MACSCIENCE
Spacegroup: P3121
Unit cell:
a: 97.856Å b: 97.856Å c: 129.601Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.197 0.196 0.269
Expression system: Escherichia coli