› 1d6a

X-ray diffraction
2.1Å resolution

STRUCTURE OF POKEWEED ANTIVIRAL PROTEIN COMPLEXED WITH GUANINE

Released:
DOI: 10.2210/pdb1d6a/pdb
PDB entry 1d6a coloured by chain, front view.
PDB entry 1d6a coloured by chain, side view.
PDB entry 1d6a coloured by chain, top view.
Source organism: Phytolacca americana
Primary publication:
X-ray crystallographic analysis of the structural basis for the interaction of pokeweed antiviral protein with guanine residues of ribosomal RNA.
Kurinov IV, Rajamohan F, Venkatachalam TK, Uckun FM
Protein Sci 8 2399-405 (1999)
PMID: 10595542

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145265 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Antiviral protein I Chains: A, B
Molecule details ›
Chains: A, B
Length: 262 amino acids
Theoretical weight: 29.34 KDa
Source organism: Phytolacca americana
UniProt:
  • Canonical: P10297 (Residues: 23-284; Coverage: 90%)
Gene name: PAP1
Sequence domains: Ribosome inactivating protein
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GUN 2 x GUN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P1
Unit cell:
a: 49.294Å b: 49.411Å c: 64.288Å
α: 68.664° β: 81.036° γ: 63.87°
R-values:
R R work R free
0.192 0.19 0.25

Quick links

Citations

5 review citations

Ribosome-inactivating and related proteins.
Schrot et al. (2015)
4 more

3 mentions without citation

Strategies to Improve the Clinical Utility of Saporin-Based Targeted Toxins.
Giansanti et al. (2018)
2 more