PDBe 1d2h

X-ray diffraction
3Å resolution

CRYSTAL STRUCTURE OF R175K MUTANT GLYCINE N-METHYLTRANSFERASE COMPLEXED WITH S-ADENOSYLHOMOCYSTEINE

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + glycine = S-adenosyl-L-homocysteine + sarcosine. 
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycine N-methyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 292 amino acids
Theoretical weight: 32.43 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P13255 (Residues: 2-293; Coverage: 100%)
Gene name: Gnmt
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P43
Unit cell:
a: 77.9Å b: 77.9Å c: 227.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.247
Expression system: Escherichia coli