› 1d0y

X-ray diffraction
2Å resolution

DICTYOSTELIUM MYOSIN S1DC (MOTOR DOMAIN FRAGMENT) COMPLEXED WITH O-NITROPHENYL AMINOETHYLDIPHOSPHATE BERYLLIUM FLUORIDE.

Released:
DOI: 10.2210/pdb1d0y/pdb
PDB entry 1d0y coloured by chain, front view.
PDB entry 1d0y coloured by chain, side view.
PDB entry 1d0y coloured by chain, top view.
Source organism: Dictyostelium discoideum
Primary publication:
X-ray structures of the Dictyostelium discoideum myosin motor domain with six non-nucleotide analogs.
Gulick AM, Bauer CB, Thoden JB, Pate E, Yount RG, Rayment I
J Biol Chem 275 398-408 (2000)
PMID: 10617631

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140379 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myosin-2 heavy chain Chain: A
Molecule details ›
Chain: A
Length: 761 amino acids
Theoretical weight: 86.74 KDa
Source organism: Dictyostelium discoideum
Expression system: Dictyostelium discoideum
UniProt:
  • Canonical: P08799 (Residues: 1-761; Coverage: 36%)
Gene names: DDB_G0286355, mhcA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ONP 1 x ONP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P21212
Unit cell:
a: 103.8Å b: 180.3Å c: 54Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.178 not available
Expression system: Dictyostelium discoideum

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Citations

1 review citation

Molecular engineering of myosin.
Manstein DJ. (2004)
20 other articles