› 1cxl

X-ray diffraction
1.81Å resolution

COMPLEX BETWEEN A COVALENT INTERMEDIATE AND BACILLUS CIRCULANS STRAIN 251 CGTASE E257Q

Released:
DOI: 10.2210/pdb1cxl/pdb
PDB entry 1cxl coloured by chain, front view.
PDB entry 1cxl coloured by chain, side view.
PDB entry 1cxl coloured by chain, top view.
Source organism: Niallia circulans
Primary publication:
X-ray structures along the reaction pathway of cyclodextrin glycosyltransferase elucidate catalysis in the alpha-amylase family.
Uitdehaag JC, Mosi R, Kalk KH, van der Veen BA, Dijkhuizen L, Withers SG, Dijkstra BW
Nat Struct Biol 6 432-6 (1999)
PMID: 10331869

Function and Biology Details

Reaction catalysed: 
Cyclizes part of a (1->4)-alpha-D-glucan chain by formation of a (1->4)-alpha-D-glucosidic bond
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155054 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Cyclomaltodextrin glucanotransferase Chain: A
Molecule details ›
Chain: A
Length: 686 amino acids
Theoretical weight: 74.56 KDa
Source organism: Niallia circulans
Expression system: Bacillus subtilis
UniProt:
  • Canonical: P43379 (Residues: 28-713; Coverage: 100%)
Gene name: cgt
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, G4D
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GLC, G4D
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GLC, G4D, GLF
Carbohydrate polymer
2 bound ligands:
Ligand CA 2 x CA
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P212121
Unit cell:
a: 117.071Å b: 109.281Å c: 65.335Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.155 0.19
Expression system: Bacillus subtilis

Quick links

Citations

29 review citations

Relationship of sequence and structure to specificity in the alpha-amylase family of enzymes.
MacGregor et al. (2001)
28 more

5 mentions without citation

Defining structural and evolutionary modules in proteins: a community detection approach to explore sub-domain architecture.
Hleap et al. (2013)
4 more