PDBe 1cw0

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE ANALYSIS OF VERY SHORT PATCH REPAIR (VSR) ENDONUCLEASE IN COMPLEX WITH A DUPLEX DNA

Released:

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
Very short patch repair protein Chain: A
Molecule details ›
Chain: A
Length: 155 amino acids
Theoretical weight: 17.91 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P09184 (Residues: 2-156; Coverage: 99%)
Gene names: JW1943, b1960, vsr
Sequence domains: DNA mismatch endonuclease Vsr
Structure domains: VSR Endonuclease
DNA (5'-D(*AP*GP*C)-3') Chain: N
Molecule details ›
Chain: N
Length: 3 nucleotides
Theoretical weight: 887 Da
Source organism: Synthetic construct
Expression system: Not provided
DNA (5'-D(P*TP*AP*GP*GP*TP*AP*CP*GP*T)-3') Chain: O
Molecule details ›
Chain: O
Length: 9 nucleotides
Theoretical weight: 2.77 KDa
Source organism: Synthetic construct
Expression system: Not provided
DNA (5'-D(*AP*CP*GP*TP*AP*CP*CP*TP*GP*GP*CP*T)-3') Chain: M
Molecule details ›
Chain: M
Length: 12 nucleotides
Theoretical weight: 3.64 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL24XU
Spacegroup: P65
Unit cell:
a: 63Å b: 63Å c: 152.5Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.204 0.23
Expression systems:
  • Escherichia coli
  • Not provided