Function and Biology

STRUCTURE OF THE MAMMALIAN CATALYTIC SUBUNIT OF CAMP-DEPENDENT PROTEIN KINASE AND AN INHIBITOR PEPTIDE DISPLAYS AN OPEN CONFORMATION

Source organisms:
Biochemical function: transferase activity
Biological process: negative regulation of protein kinase activity
Cellular component: germinal vesicle

EC 2.7.11.11: cAMP-dependent protein kinase

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein.
Comments:
  • cAMP is required to activate this enzyme.
  • The inactive holoenzyme of cAMP-dependent protein kinase is a tetramer composed of two regulatory (R) and two catalytic (C) subunits.
  • cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP molecules and two free monomeric catalytic subunits (i.e. R(2)C(2) + 4 cAMP = R(2)(cAMP)(4) + 2 C).
  • Formerly EC 2.7.1.37.
Systematic name:
ATP:protein phosphotransferase (cAMP-dependent)
Alternative Name(s):
  • PKA
  • PKA C
  • Protein kinase A
  • STK22

Sequence family

Pfam Protein family (Pfam)
PF00069
Domain description: Protein kinase domain
Occurring in:
  1. cAMP-dependent protein kinase catalytic subunit alpha
1 copy of Pfam domain PF00069 (Protein kinase domain) in cAMP-dependent protein kinase catalytic subunit alpha in PDB 1ctp.

InterPro InterPro annotations
IPR004171
Domain description: cAMP-dependent protein kinase inhibitor
Occurring in:
  1. cAMP-dependent protein kinase inhibitor alpha
IPR000961
Domain description: AGC-kinase, C-terminal
Occurring in:
  1. cAMP-dependent protein kinase catalytic subunit alpha
IPR000719
Domain description: Protein kinase domain
Occurring in:
  1. cAMP-dependent protein kinase catalytic subunit alpha
IPR017441
Domain description: Protein kinase, ATP binding site
Occurring in:
  1. cAMP-dependent protein kinase catalytic subunit alpha
IPR008271
Domain description: Serine/threonine-protein kinase, active site
Occurring in:
  1. cAMP-dependent protein kinase catalytic subunit alpha
IPR011009
Domain description: Protein kinase-like domain superfamily
Occurring in:
  1. cAMP-dependent protein kinase catalytic subunit alpha

Structure domains

CATH CATH domains
1.10.510.10
Class: Mainly Alpha
Architecture: Orthogonal Bundle
Topology: Transferase(Phosphotransferase); domain 1
Homology: Transferase(Phosphotransferase) domain 1
Occurring in:
  1. cAMP-dependent protein kinase catalytic subunit alpha
1 copy of CATH domain 1.10.510.10 (Transferase(Phosphotransferase); domain 1) in cAMP-dependent protein kinase catalytic subunit alpha in PDB 1ctp.
3.30.200.20
Class: Alpha Beta
Architecture: 2-Layer Sandwich
Topology: Phosphorylase Kinase; domain 1
Homology: Phosphorylase Kinase; domain 1
Occurring in:
  1. cAMP-dependent protein kinase catalytic subunit alpha
1 copy of CATH domain 3.30.200.20 (Phosphorylase Kinase; domain 1) in cAMP-dependent protein kinase catalytic subunit alpha in PDB 1ctp.
SCOP SCOP annotation
88854
Class: Alpha and beta proteins (a+b)
Fold: Protein kinase-like (PK-like)
Superfamily: Protein kinase-like (PK-like)
Occurring in:
  1. cAMP-dependent protein kinase catalytic subunit alpha
1 copy of SCOP domain 88854 (Protein kinases, catalytic subunit) in cAMP-dependent protein kinase catalytic subunit alpha in PDB 1ctp.