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CRYSTAL STRUCTURE OF CHOLESTEROL OXIDASE COMPLEXED WITH A STEROID SUBSTRATE. IMPLICATIONS FOR FAD DEPENDENT ALCOHOL OXIDASES

Source organism: Brevibacterium sterolicum
Biochemical function: flavin adenine dinucleotide binding
Biological process: cholesterol metabolic process
Cellular component: extracellular region

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EC 5.3.3.1: Steroid Delta-isomerase

Reaction catalysed:
A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid
Systematic name:
3-oxosteroid Delta(5)-Delta(4)-isomerase
Alternative Name(s):
  • 3-oxosteroid isomerase
  • Delta(5)(or Delta(4))-3-keto steroid isomerase
  • Delta(5)-3-keto steroid isomerase
  • Delta(5)-3-ketosteroid isomerase
  • Delta(5)-3-oxosteroid isomerase
  • Delta(5)-ketosteroid isomerase
  • Delta(5)-steroid isomerase
  • Hydroxysteroid isomerase
  • Steroid isomerase

EC 1.1.3.6: Cholesterol oxidase

Reaction catalysed:
Cholesterol + O(2) = cholest-5-en-3-one + H(2)O(2)
Systematic name:
Cholesterol:oxygen oxidoreductase
Alternative Name(s):
  • 3-beta-hydroxy steroid oxidoreductase
  • 3-beta-hydroxysteroid:oxygen oxidoreductase
  • Cholesterol-O(2) oxidoreductase

GO terms

Sequence families

Pfam Protein families (Pfam)
PF05199
Domain description: GMC oxidoreductase
Occurring in:
  1. Cholesterol oxidase
The deposited structure of PDB entry 1coy contains 1 copy of Pfam domain PF05199 (GMC oxidoreductase) in Cholesterol oxidase. Showing 1 copy in chain A.

PF00732
Domain description: GMC oxidoreductase
Occurring in:
  1. Cholesterol oxidase
The deposited structure of PDB entry 1coy contains 1 copy of Pfam domain PF00732 (GMC oxidoreductase) in Cholesterol oxidase. Showing 1 copy in chain A.

InterPro InterPro annotations
IPR000172
Domain description: Glucose-methanol-choline oxidoreductase, N-terminal
Occurring in:
  1. Cholesterol oxidase
IPR036188
Domain description: FAD/NAD(P)-binding domain superfamily
Occurring in:
  1. Cholesterol oxidase
IPR007867
Domain description: Glucose-methanol-choline oxidoreductase, C-terminal
Occurring in:
  1. Cholesterol oxidase

Structure domains

CATH CATH domains
3.50.50.60
Class: Alpha Beta
Architecture: 3-Layer(bba) Sandwich
Topology: FAD/NAD(P)-binding domain
Homology: FAD/NAD(P)-binding domain
Occurring in:
  1. Cholesterol oxidase
The deposited structure of PDB entry 1coy contains 1 copy of CATH domain 3.50.50.60 (FAD/NAD(P)-binding domain) in Cholesterol oxidase. Showing 1 copy in chain A.
3.30.410.10
Class: Alpha Beta
Architecture: 2-Layer Sandwich
Topology: Cholesterol Oxidase; domain 2
Homology: Cholesterol Oxidase; domain 2
Occurring in:
  1. Cholesterol oxidase
The deposited structure of PDB entry 1coy contains 1 copy of CATH domain 3.30.410.10 (Cholesterol Oxidase; domain 2) in Cholesterol oxidase. Showing 1 copy in chain A.
SCOP SCOP annotations
51913
Class: Alpha and beta proteins (a/b)
Fold: FAD/NAD(P)-binding domain
Superfamily: FAD/NAD(P)-binding domain
Occurring in:
  1. Cholesterol oxidase
The deposited structure of PDB entry 1coy contains 1 copy of SCOP domain 51913 (FAD-linked reductases, N-terminal domain) in Cholesterol oxidase. Showing 1 copy in chain A.
54374
Class: Alpha and beta proteins (a+b)
Fold: FAD-linked reductases, C-terminal domain
Superfamily: FAD-linked reductases, C-terminal domain
Occurring in:
  1. Cholesterol oxidase
The deposited structure of PDB entry 1coy contains 1 copy of SCOP domain 54374 (GMC oxidoreductases) in Cholesterol oxidase. Showing 1 copy in chain A.

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