PDBe 1cml

X-ray diffraction
1.69Å resolution

CHALCONE SYNTHASE FROM ALFALFA COMPLEXED WITH MALONYL-COA

Released:
Source organism: Medicago sativa

Function and Biology Details

Reaction catalysed:
3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO(2). 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chalcone synthase 2 Chain: A
Molecule details ›
Chain: A
Length: 389 amino acids
Theoretical weight: 42.72 KDa
Source organism: Medicago sativa
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P30074 (Residues: 1-389; Coverage: 100%)
Gene name: CHS2
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MACSCIENCE SRA
Spacegroup: P3221
Unit cell:
a: 97.768Å b: 97.768Å c: 65.228Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.167 0.167 0.2
Expression system: Escherichia coli BL21