PDBe 1clv

X-ray diffraction
2Å resolution

YELLOW MEAL WORM ALPHA-AMYLASE IN COMPLEX WITH THE AMARANTH ALPHA-AMYLASE INHIBITOR

Released:

Function and Biology Details

Reaction catalysed:
Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Alpha-amylase Chain: A
Molecule details ›
Chain: A
Length: 471 amino acids
Theoretical weight: 51.26 KDa
Source organism: Tenebrio molitor
UniProt:
  • Canonical: P56634 (Residues: 1-471; Coverage: 100%)
Sequence domains:
Structure domains:
Alpha-amylase inhibitor AAI Chain: I
Molecule details ›
Chain: I
Length: 32 amino acids
Theoretical weight: 3.6 KDa
Source organism: Amaranthus hypochondriacus
Expression system: Not provided
UniProt:
  • Canonical: P80403 (Residues: 1-32; Coverage: 100%)
Gene name: AAI

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P61
Unit cell:
a: 119.25Å b: 119.25Å c: 64.78Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.161 0.161 0.19
Expression system: Not provided