PDBe 1ckq

X-ray diffraction
1.85Å resolution

PRE-TRANSITION STATE ECO RI ENDONUCLEASE/COGNATE DNA (TCGCGAATTCGCG) COMPLEX

Released:
Entry authors: Horvath M, Rosenberg JM

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates. 
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Type-2 restriction enzyme EcoRI Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 30.97 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P00642 (Residues: 2-277; Coverage: 100%)
Gene name: ecoRIR
Sequence domains: Restriction endonuclease EcoRI
Structure domains: Eco RI Endonuclease, subunit A
DNA (5'-D(*TP*CP*GP*CP*GP*AP*AP*TP*TP*CP*GP*CP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 13 nucleotides
Theoretical weight: 3.97 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P321
Unit cell:
a: 118.11Å b: 118.11Å c: 49.45Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.235 0.235 0.286
Expression system: Not provided