PDBe 1cju

X-ray diffraction
2.8Å resolution

COMPLEX OF GS-ALPHA WITH THE CATALYTIC DOMAINS OF MAMMALIAN ADENYLYL CYCLASE: COMPLEX WITH BETA-L-2',3'-DIDEOXYATP AND MG

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Adenylate cyclase type 5 Chain: A
Molecule details ›
Chain: A
Length: 217 amino acids
Theoretical weight: 24.5 KDa
Source organism: Canis lupus familiaris
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P30803 (Residues: 445-661; Coverage: 17%)
Gene name: ADCY5
Sequence domains: Adenylate and Guanylate cyclase catalytic domain
Structure domains: Adenylyl Cyclase, chain A
Adenylate cyclase type 2 Chain: B
Molecule details ›
Chain: B
Length: 212 amino acids
Theoretical weight: 23.72 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P26769 (Residues: 870-1081; Coverage: 19%)
Gene name: Adcy2
Sequence domains: Adenylate and Guanylate cyclase catalytic domain
Structure domains: Adenylyl Cyclase, chain A
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: C
Molecule details ›
Chain: C
Length: 402 amino acids
Theoretical weight: 46.66 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04896 (Residues: 1-70, 71-394; Coverage: 100%)
Gene names: GNAS, GNAS1
Sequence domains: G-protein alpha subunit
Structure domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P21212
Unit cell:
a: 118.3Å b: 134.2Å c: 71.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.222 0.284
Expression system: Escherichia coli BL21(DE3)