PDBe 1cit

X-ray diffraction
2.7Å resolution

DNA-BINDING MECHANISM OF THE MONOMERIC ORPHAN NUCLEAR RECEPTOR NGFI-B

Released:
Primary publication:
DNA-binding mechanism of the monomeric orphan nuclear receptor NGFI-B.
Nat. Struct. Biol. 6 471-7 (1999)
PMID: 10331876

Function and Biology Details

Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Nuclear receptor subfamily 4 group A member 1 Chain: A
Molecule details ›
Chain: A
Length: 89 amino acids
Theoretical weight: 10.1 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22829 (Residues: 264-352; Coverage: 15%)
Gene names: Hmr, Ngfib, Nr4a1
Sequence domains: Zinc finger, C4 type (two domains)
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
DNA (5'-D(*CP*CP*GP*AP*AP*AP*AP*GP*GP*TP*CP*AP*TP*GP*CP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 4.93 KDa
Source organism: Synthetic construct
Expression system: Not provided
DNA (5'-D(*CP*GP*CP*AP*TP*GP*AP*CP*CP*TP*TP*TP*TP*CP*GP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.87 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P21
Unit cell:
a: 53.42Å b: 34.24Å c: 56.26Å
α: 90° β: 109.13° γ: 90°
R-values:
R R work R free
0.219 0.219 0.295
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided