PDBe 1chk

X-ray diffraction
2.4Å resolution

STREPTOMYCES N174 CHITOSANASE PH5.5 298K

Released:
Source organism: Streptomyces sp. N174

Function and Biology Details

Reaction catalysed:
Endohydrolysis of beta-(1->4)-linkages between D-glucosamine residues in a partly acetylated chitosan. 
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chitosanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 238 amino acids
Theoretical weight: 25.85 KDa
Source organism: Streptomyces sp. N174
Expression system: Streptomyces lividans
UniProt:
  • Canonical: P33665 (Residues: 41-278; Coverage: 100%)
Gene names: chs, csn
Sequence domains: Glycosyl hydrolase family 46
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 56.3Å b: 59.3Å c: 85.4Å
α: 90° β: 96.2° γ: 90°
R-values:
R R work R free
0.181 0.181 0.175
Expression system: Streptomyces lividans