PDBe 1cfr

X-ray diffraction
2.15Å resolution

CRYSTAL STRUCTURE OF CITROBACTER FREUNDII RESTRICTION ENDONUCLEASE CFR10I AT 2.15 ANGSTROMS RESOLUTION.

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates. 
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type-2 restriction enzyme Cfr10I Chain: A
Molecule details ›
Chain: A
Length: 285 amino acids
Theoretical weight: 32.05 KDa
Source organism: Citrobacter freundii
Expression system: Escherichia coli
UniProt:
  • Canonical: P56200 (Residues: 1-285; Coverage: 100%)
Gene name: cfr10IR
Sequence domains: Cfr10I/Bse634I restriction endonuclease
Structure domains: Restriction Endonuclease

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: I222
Unit cell:
a: 64.5Å b: 81.3Å c: 119.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 not available
Expression system: Escherichia coli