PDBe 1cf4

Solution NMR

CDC42/ACK GTPASE-BINDING DOMAIN COMPLEX

Released:

Function and Biology Details

Reactions catalysed:
ATP + a protein = ADP + a phosphoprotein. 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. 
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cell division control protein 42 homolog Chain: A
Molecule details ›
Chain: A
Length: 184 amino acids
Theoretical weight: 20.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P60953 (Residues: 1-184; Coverage: 96%)
Gene name: CDC42
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Activated CDC42 kinase 1 Chain: B
Molecule details ›
Chain: B
Length: 44 amino acids
Theoretical weight: 4.84 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q07912 (Residues: 446-489; Coverage: 4%)
Gene names: ACK1, TNK2
Structure domains: Activated P21cdc42hs Kinase; Chain B

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 63%
Refinement method: DISTANCE GEOMETRY, SIMULATED ANNEALING
Chemical shifts: BMR4700  
Expression system: Escherichia coli