PDBe 1cen

X-ray diffraction
2.3Å resolution

CELLULASE (CELC) MUTANT WITH GLU 140 REPLACED BY GLN COMPLEXED WITH CELLOHEXAOSE

Released:

Function and Biology Details

Reaction catalysed:
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans. 
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase C Chain: A
Molecule details ›
Chain: A
Length: 343 amino acids
Theoretical weight: 40.95 KDa
Source organism: Ruminiclostridium thermocellum
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C2S3 (Residues: 1-343; Coverage: 100%)
Gene name: celC
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P212121
Unit cell:
a: 51.4Å b: 84.33Å c: 87.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.178 0.246
Expression system: Escherichia coli