› 1cel

X-ray diffraction
1.8Å resolution

THE THREE-DIMENSIONAL CRYSTAL STRUCTURE OF THE CATALYTIC CORE OF CELLOBIOHYDROLASE I FROM TRICHODERMA REESEI

Released:
DOI: 10.2210/pdb1cel/pdb
PDB entry 1cel coloured by chain, front view.
PDB entry 1cel coloured by chain, side view.
PDB entry 1cel coloured by chain, top view.
Source organism: Trichoderma reesei
Primary publication:
The three-dimensional crystal structure of the catalytic core of cellobiohydrolase I from Trichoderma reesei.
Divne C, Ståhlberg J, Reinikainen T, Ruohonen L, Pettersson G, Knowles JK, Teeri TT, Jones TA
Science 265 524-8 (1994)
PMID: 8036495

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158661 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Exoglucanase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 434 amino acids
Theoretical weight: 46.01 KDa
Source organism: Trichoderma reesei
Expression system: Not provided
UniProt:
  • Canonical: P62694 (Residues: 18-451; Coverage: 88%)
Gene name: cbh1
Sequence domains: Glycosyl hydrolase family 7
Structure domains: Glycoside hydrolase, family 7, domain

Ligands and Environments

5 bound ligands:
Ligand NAG 2 x NAG
Ligand BGC 1 x BGC
Ligand CA 1 x CA
Ligand IBZ 2 x IBZ
Ligand GLC 1 x GLC
1 modified residue:
Ligand PCA 2 x PCA

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21212
Unit cell:
a: 84Å b: 86.2Å c: 111.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.181 not available
Expression system: Not provided

Quick links

Citations

27 review citations

Microbial cellulose utilization: fundamentals and biotechnology.
Lynd et al. (2002)
26 more

24 mentions without citation

Lignocellulose degradation mechanisms across the Tree of Life.
Cragg et al. (2015)
23 more