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Structures of apo and complexed Escherichia coli glycinamide ribonucleotide transformylase.

Almassy RJ, Janson CA, Kan CC, Hostomska Z
Proc Natl Acad Sci U S A 89 6114-8 (1992)
Cited: 60 times
EuropePMC logo PMID: 1631098

Abstract

The three-dimensional structure of phosphoribosylglycinamide formyltransferase (10-formyltetrahydrofolate:5'-phosphoribosylglycinamide formyltransferase, EC 2.1.2.2) has been solved both as an apoenzyme at 2.8-A resolution and as a ternary complex with the substrate glycinamide ribonucleotide and a folate inhibitor at 2.5-A resolution. The structure is a modified doubly wound alpha/beta sheet with flexibility in the active site, including a disordered loop in the apo structure, which is ordered in the ternary complex structure. This enzyme is a target for anti-cancer therapy and now for structure-based drug design.

Reviews - 1cde mentioned but not cited (1)

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Articles - 1cde mentioned but not cited (12)

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Reviews citing this publication (5)

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  38. Identification of Active Site Residues of the Siderophore Synthesis Enzyme PvdF and Evidence for Interaction of PvdF with a Substrate-Providing Enzyme. Philem P, Kleffmann T, Gai S, Hawkins BC, Wilbanks SM, Lamont IL. Int J Mol Sci 22 2211 (2021)
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  41. Stereoselective Synthesis of β-Glycinamide Ribonucleotide. Ngu L, Ray D, Watson SS, Beuning PJ, Ondrechen MJ, O'Doherty GA. Molecules 27 2528 (2022)
  42. Structural features of Cryptococcus neoformans bifunctional GAR/AIR synthetase may present novel antifungal drug targets. Chua SMH, Wizrah MSI, Luo Z, Lim BYJ, Kappler U, Kobe B, Fraser JA. J Biol Chem 297 101091 (2021)


Related citations provided by authors (2)

  1. De Novo Purine Nucleotide Biosynthesis: Cloning of Human and Avian Cdna'S Encoding the Trifunctional Glycinamide Ribonucleotide Synthetase-Aminoimidazole Ribonucleotide Synthetase-Glycinamide Ribonucleotide Transformylase by Functional Complementation in E. Coli. Aimi J, Qiu H, Williams J, Zalkin H, Dixon JE Nucleic Acids Res. 18 6665- (1990)
  2. Identification and Nucleotide Sequence of a Gene Encoding 5'-Phosphoribosylglycinamide Transformylase in Escherichia Coli K12. Smith JM, Daum /III HA J. Biol. Chem. 262 10565- (1987)

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