PDBe 1cc1

X-ray diffraction
2.15Å resolution

CRYSTAL STRUCTURE OF A REDUCED, ACTIVE FORM OF THE NI-FE-SE HYDROGENASE FROM DESULFOMICROBIUM BACULATUM

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Periplasmic [NiFeSe] hydrogenase large subunit Chain: L
Molecule details ›
Chain: L
Length: 498 amino acids
Theoretical weight: 55.22 KDa
Source organism: Desulfomicrobium baculatum
UniProt:
  • Canonical: P13065 (Residues: 2-499; Coverage: 97%)
Sequence domains: Nickel-dependent hydrogenase
Structure domains: Cytochrome-c3 Hydrogenase, chain B
Periplasmic [NiFeSe] hydrogenase small subunit Chain: S
Molecule details ›
Chain: S
Length: 283 amino acids
Theoretical weight: 30.89 KDa
Source organism: Desulfomicrobium baculatum
UniProt:
  • Canonical: P13063 (Residues: 33-315; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE D2AM
Spacegroup: P212121
Unit cell:
a: 110.39Å b: 63.7Å c: 99.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.194 0.248