Function and Biology

STRUCTURE OF TNF RECEPTOR ASSOCIATED FACTOR 2 IN COMPLEX WITH A PEPTIDE FROM TNF-R2

Source organism: Homo sapiens
Biochemical function: ubiquitin-protein transferase activity
Biological process: protein autoubiquitination
Cellular component: not assigned

EC 2.3.2.27: RING-type E3 ubiquitin transferase

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)- ubiquitinyl-[acceptor protein]-L-lysine.
Comments:
  • The RING domain of E3 ubiquitin transferase serves as a mediator bringing the ubiquitin-charged E2 ubiquitin-conjugating enzyme and the acceptor protein together to enable the direct transfer of ubiquitin through the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin an the epsilon-amino group of an L-lysine residue of the acceptor protein.
  • The RING-E3 domain does not form a catalytic thioester intermediate with ubiquitin (unlike the HECT domain, EC 2.3.2.26).
  • RING-type ubiquitin transferases may occur as single-chain enzymes but also in dimeric forms or in multi-subunit assemblies.
  • Formerly EC 6.3.2.19 and EC 6.3.2.21.
Systematic name:
S-ubiquitinyl-[ubiquitin-conjugating E2 enzyme]-L-cysteine:acceptor protein ubiquitin transferase (isopeptide bond-forming; RING-type)
Alternative Name(s):
  • RING E3 ligase
  • Ubiquitin transferase RING E3

Sequence family

InterPro InterPro annotations
IPR002083
Domain description: MATH/TRAF domain
Occurring in:
  1. TNF receptor-associated factor 2
IPR008974
Domain description: TRAF-like
Occurring in:
  1. TNF receptor-associated factor 2
IPR037305
Domain description: TNF receptor-associated factor 2, MATH domain
Occurring in:
  1. TNF receptor-associated factor 2
IPR027133
Domain description: TNF receptor-associated factor 2
Occurring in:
  1. TNF receptor-associated factor 2

Structure domain

CATH CATH domain
2.60.210.10
Class: Mainly Beta
Architecture: Sandwich
Topology: Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain A
Homology: Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain A
Occurring in:
  1. TNF receptor-associated factor 2
1 copy of CATH domain 2.60.210.10 (Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain A) in TNF receptor-associated factor 2 in PDB 1ca9.
SCOP SCOP annotations
49600
Class: All beta proteins
Fold: TRAF domain-like
Superfamily: TRAF domain-like
Occurring in:
  1. TNF receptor-associated factor 2
1 copy of SCOP domain 49600 (MATH domain) in TNF receptor-associated factor 2 in PDB 1ca9.
57954
Class: Coiled coil proteins
Fold: Parallel coiled-coil
Superfamily: Trimerization domain of TRAF
Occurring in:
  1. TNF receptor-associated factor 2
1 copy of SCOP domain 57954 (Trimerization domain of TRAF) in TNF receptor-associated factor 2 in PDB 1ca9.