EC 188.8.131.52: RING-type E3 ubiquitin transferase
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)- ubiquitinyl-[acceptor protein]-L-lysine.
- The RING domain of E3 ubiquitin transferase serves as a mediator bringing the ubiquitin-charged E2 ubiquitin-conjugating enzyme and the acceptor protein together to enable the direct transfer of ubiquitin through the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin an the epsilon-amino group of an L-lysine residue of the acceptor protein.
- The RING-E3 domain does not form a catalytic thioester intermediate with ubiquitin (unlike the HECT domain, EC 184.108.40.206).
- RING-type ubiquitin transferases may occur as single-chain enzymes but also in dimeric forms or in multi-subunit assemblies.
- Formerly EC 220.127.116.11 and EC 18.104.22.168.
S-ubiquitinyl-[ubiquitin-conjugating E2 enzyme]-L-cysteine:acceptor protein ubiquitin transferase (isopeptide bond-forming; RING-type)
- RING E3 ligase
- Ubiquitin transferase RING E3
Domain description: MATH/TRAF domain
Domain description: TRAF-like
Domain description: TNF receptor-associated factor 2, MATH domain
Domain description: TNF receptor-associated factor 2
Class: Mainly Beta
Topology: Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain A
Homology: Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain AOccurring in: