PDBe 1ca9

X-ray diffraction
2.3Å resolution

STRUCTURE OF TNF RECEPTOR ASSOCIATED FACTOR 2 IN COMPLEX WITH A PEPTIDE FROM TNF-R2

Released:
Source organism: Homo sapiens

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)- ubiquitinyl-[acceptor protein]-L-lysine. 
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero pentamer (preferred)
homo trimer
hetero octamer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tumor necrosis factor-binding protein 2 Chains: G, H
Molecule details ›
Chains: G, H
Length: 10 amino acids
Theoretical weight: 1.12 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P20333 (Residues: 420-428; Coverage: 2%)
Gene names: TNFBR, TNFR2, TNFRSF1B
TNF receptor-associated factor 2 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 192 amino acids
Theoretical weight: 21.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q12933 (Residues: 310-501; Coverage: 38%)
Gene names: TRAF2, TRAP3
Structure domains: Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain A

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21
Unit cell:
a: 83Å b: 84.4Å c: 100.7Å
α: 90° β: 108.7° γ: 90°
R-values:
R R work R free
0.234 0.234 0.289
Expression systems:
  • Not provided
  • Escherichia coli BL21