› 1ca7

X-ray diffraction
2.5Å resolution

MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF) WITH HYDROXPHENYLPYRUVATE

Released:
DOI: 10.2210/pdb1ca7/pdb
PDB entry 1ca7 coloured by chain, front view.
PDB entry 1ca7 coloured by chain, side view.
PDB entry 1ca7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Pro-1 of macrophage migration inhibitory factor functions as a catalytic base in the phenylpyruvate tautomerase activity.
Lubetsky JB, Swope M, Dealwis C, Blake P, Lolis E
Biochemistry 38 7346-54 (1999)
PMID: 10353846

Function and Biology Details

Reactions catalysed: 
Keto-phenylpyruvate = enol-phenylpyruvate
L-dopachrome = 5,6-dihydroxyindole-2-carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146863 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Macrophage migration inhibitory factor Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 114 amino acids
Theoretical weight: 12.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14174 (Residues: 2-115; Coverage: 99%)
Gene names: GLIF, MIF, MMIF
Sequence domains: Macrophage migration inhibitory factor (MIF)
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

1 bound ligand:
Ligand ENO 3 x ENO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 67.85Å b: 68.04Å c: 87.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.2 0.263
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

MIF: a new cytokine link between rheumatoid arthritis and atherosclerosis.
Morand et al. (2006)
16 more

16 mentions without citation

Small-molecule inhibitors of macrophage migration inhibitory factor (MIF) as an emerging class of therapeutics for immune disorders.
Kok et al. (2018)
15 more