1c9u Citations

Structure and mechanism of soluble quinoprotein glucose dehydrogenase.

EMBO J. 18 5187-94 (1999)
Cited: 1 times
EuropePMC logo PMID: 10508152

Abstract

Soluble glucose dehydrogenase (s-GDH; EC 1.1.99.17) is a classical quinoprotein which requires the cofactor pyrroloquinoline quinone (PQQ) to oxidize glucose to gluconolactone. The reaction mechanism of PQQ-dependent enzymes has remained controversial due to the absence of comprehensive structural data. We have determined the X-ray structure of s-GDH with the cofactor at 2.2 A resolution, and of a complex with reduced PQQ and glucose at 1.9 A resolution. These structures reveal the active site of s-GDH, and show for the first time how a functionally bound substrate interacts with the cofactor in a PQQ-dependent enzyme. Twenty years after the discovery of PQQ, our results finally provide conclusive evidence for a reaction mechanism comprising general base-catalyzed hydride transfer, rather than the generally accepted covalent addition-elimination mechanism. Thus, PQQ-dependent enzymes use a mechanism similar to that of nicotinamide- and flavin-dependent oxidoreductases.

Articles - 1c9u mentioned but not cited (1)

  1. A Nernstian Biosupercapacitor. Pankratov D, Conzuelo F, Pinyou P, Alsaoub S, Schuhmann W, Shleev S. Angew. Chem. Int. Ed. Engl. 55 15434-15438 (2016)




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