Function and Biology

CRYSTAL STRUCTURE OF THE SOLUBLE QUINOPROTEIN GLUCOSE DEHYDROGENASE IN COMPLEX WITH PQQ

Source organism: Acinetobacter calcoaceticus
Biochemical function: metal ion binding
Biological process: oxidation-reduction process
Cellular component: not assigned

EC 1.1.5.2: Quinoprotein glucose dehydrogenase (PQQ, quinone)

Reaction catalysed:
D-glucose + ubiquinone = D-glucono-1,5-lactone + ubiquinol.
Comments:
  • Integral membrane protein containing PQQ as prosthetic group.
  • It also contains bound ubiquinone and Mg(2+) or Ca(2+).
  • Electron acceptor is membrane ubiquinone but usually assayed with phenazine methosulfate.
  • Like in all other quinoprotein alcohol dehydrogenases the catalytic domain has an 8-bladed 'propeller' structure.
  • It occurs in a wide range of bacteria.
  • Catalyzes a direct oxidation of the pyranose form of D-glucose to the lactone and thence to D-gluconate in the periplasm.
  • Oxidizes other monosaccharides including the pyranose forms of pentoses.
  • Formerly EC 1.1.99.17.
Systematic name:
D-glucose:ubiquinone oxidoreductase
Alternative Name(s):
  • D-glucose:(pyrroloquinoline-quinone) 1-oxidoreductase
  • Glucose dehydrogenase (PQQ-dependent)
  • Glucose dehydrogenase (pyrroloquinoline-quinone)
  • Quinoprotein D-glucose dehydrogenase
  • Membrane-bound glucose dehydrogenase
  • MGDH

GO terms

Sequence family

CATH Protein family (Pfam)
PF07995
Domain description: Glucose / Sorbosone dehydrogenase
Occurring in:
  1. Quinoprotein glucose dehydrogenase B
2 copies of Pfam domain PF07995 (Glucose / Sorbosone dehydrogenase) in Quinoprotein glucose dehydrogenase B in PDB 1c9u.

CATH InterPro annotations
IPR012938
Domain description: Glucose/Sorbosone dehydrogenase
Occurring in:
  1. Quinoprotein glucose dehydrogenase B
IPR019893
Domain description: PQQ-dependent dehydrogenase, s-GDH family
Occurring in:
  1. Quinoprotein glucose dehydrogenase B
IPR011041
Domain description: Soluble quinoprotein glucose/sorbosone dehydrogenase
Occurring in:
  1. Quinoprotein glucose dehydrogenase B
IPR011042
Domain description: Six-bladed beta-propeller, TolB-like
Occurring in:
  1. Quinoprotein glucose dehydrogenase B

Structure domain

CATH CATH domain
2.120.10.30
Class: Mainly Beta
Architecture: 6 Propellor
Topology: Neuraminidase
Homology: TolB, C-terminal domain
Occurring in:
  1. Quinoprotein glucose dehydrogenase B
1 copy of CATH domain 2.120.10.30 (Neuraminidase) in Quinoprotein glucose dehydrogenase B in PDB 1c9u.
SCOP SCOP annotation
50953
Class: All beta proteins
Fold: 6-bladed beta-propeller
Superfamily: Soluble quinoprotein glucose dehydrogenase
Occurring in:
  1. Quinoprotein glucose dehydrogenase B
1 copy of SCOP domain 50953 (Soluble quinoprotein glucose dehydrogenase) in Quinoprotein glucose dehydrogenase B in PDB 1c9u.