PDBe 1c4x

X-ray diffraction
2.4Å resolution

2-HYDROXY-6-OXO-6-PHENYLHEXA-2,4-DIENOATE HYDROLASE (BPHD) FROM RHODOCOCCUS SP. STRAIN RHA1

Released:

Function and Biology Details

Reaction catalysed:
2,6-dioxo-6-phenylhexa-3-enoate + H(2)O = benzoate + 2-oxopent-4-enoate. 
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase Chain: A
Molecule details ›
Chain: A
Length: 285 amino acids
Theoretical weight: 31.68 KDa
Source organism: Rhodococcus jostii RHA1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q75WN8 (Residues: 1-285; Coverage: 100%)
Gene names: RHA1_ro10136, bphD, bphD1
Sequence domains:
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: I422
Unit cell:
a: 110.8Å b: 110.8Å c: 136.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.178 0.227
Expression system: Escherichia coli