PDBe 1c3j

X-ray diffraction
1.88Å resolution

T4 PHAGE BETA-GLUCOSYLTRANSFERASE: SUBSTRATE BINDING AND PROPOSED CATALYTIC MECHANISM

Released:

Function and Biology Details

Reaction catalysed:
Transfers a beta-D-glucosyl residue from UDP-alpha-D-glucose to a hydroxymethylcytosine residue in DNA. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA beta-glucosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 351 amino acids
Theoretical weight: 40.72 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P04547 (Residues: 1-351; Coverage: 100%)
Gene name: bgt
Sequence domains: Bacteriophage T4 beta-glucosyltransferase
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE BEAMLINE DW32
Spacegroup: P21212
Unit cell:
a: 151.7Å b: 52.26Å c: 52.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.191 0.233
Expression system: Escherichia coli