PDBe 1bwp

X-ray diffraction
2.1Å resolution

PROBING THE SUBSTRATE SPECIFICITY OF THE INTRACELLULAR BRAIN PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE

Released:

Function and Biology Details

Reaction catalysed:
1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H(2)O = 1-alkyl- sn-glycero-3-phosphocholine + acetate. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Platelet-activating factor acetylhydrolase IB subunit gamma Chain: A
Molecule details ›
Chain: A
Length: 233 amino acids
Theoretical weight: 25.96 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q29460 (Residues: 1-232; Coverage: 100%)
Gene names: PAFAH1B3, PAFAHG
Sequence domains: GDSL-like Lipase/Acylhydrolase family
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P3121
Unit cell:
a: 81.8Å b: 81.8Å c: 72.98Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.209 0.252
Expression system: Escherichia coli BL21