PDBe 1buh

X-ray diffraction
2.6Å resolution

CRYSTAL STRUCTURE OF THE HUMAN CDK2 KINASE COMPLEX WITH CELL CYCLE-REGULATORY PROTEIN CKSHS1

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cyclin-dependent kinase 2 Chain: A
Molecule details ›
Chain: A
Length: 298 amino acids
Theoretical weight: 33.98 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P24941 (Residues: 1-298; Coverage: 100%)
Gene names: CDK2, CDKN2
Sequence domains: Protein kinase domain
Structure domains:
Cyclin-dependent kinases regulatory subunit 1 Chain: B
Molecule details ›
Chain: B
Length: 79 amino acids
Theoretical weight: 9.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61024 (Residues: 1-79; Coverage: 100%)
Gene names: CKS1, CKS1B, PNAS-143, PNAS-16
Sequence domains: Cyclin-dependent kinase regulatory subunit
Structure domains: Cell cycle regulatory proteins

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SIEMENS
Spacegroup: P21
Unit cell:
a: 56.8Å b: 69.95Å c: 60.53Å
α: 90° β: 114.5° γ: 90°
R-values:
R R work R free
0.19 0.19 0.25
Expression systems:
  • Trichoplusia ni
  • Escherichia coli