PDBe 1bou

X-ray diffraction
2.2Å resolution

THREE-DIMENSIONAL STRUCTURE OF LIGAB

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protocatechuate 4,5-dioxygenase beta chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 302 amino acids
Theoretical weight: 33.32 KDa
Source organism: Sphingomonas paucimobilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P22636 (Residues: 1-302; Coverage: 100%)
Gene name: ligB
Sequence domains: Catalytic LigB subunit of aromatic ring-opening dioxygenase
Structure domains: LigB-like
Protocatechuate 4,5-dioxygenase alpha chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 139 amino acids
Theoretical weight: 15.57 KDa
Source organism: Sphingomonas paucimobilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P22635 (Residues: 1-139; Coverage: 100%)
Gene name: ligA
Sequence domains: Aromatic-ring-opening dioxygenase LigAB, LigA subunit
Structure domains: LigA subunit of an aromatic-ring-opening dioxygenase LigAB

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P21
Unit cell:
a: 65.4Å b: 66.5Å c: 119.8Å
α: 90° β: 92.5° γ: 90°
R-values:
R R work R free
0.149 0.149 0.206
Expression system: Escherichia coli