PDBe 1boo

X-ray diffraction
2.8Å resolution

PVUII DNA METHYLTRANSFERASE (CYTOSINE-N4-SPECIFIC)

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Modification methylase PvuII Chain: A
Molecule details ›
Chain: A
Length: 323 amino acids
Theoretical weight: 36.96 KDa
Source organism: Proteus vulgaris
Expression system: Escherichia coli str. K-12 substr. DH10B
UniProt:
  • Canonical: P11409 (Residues: 14-336; Coverage: 96%)
Gene name: pvuIIM
Sequence domains: DNA methylase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SAH 1 x SAH
No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P21
Unit cell:
a: 48.8Å b: 112.4Å c: 59.3Å
α: 90° β: 109.2° γ: 90°
R-values:
R R work R free
0.193 0.193 0.283
Expression system: Escherichia coli str. K-12 substr. DH10B