PDBe 1bm2

X-ray diffraction
2.1Å resolution

GRB2-SH2 DOMAIN IN COMPLEX WITH CYCLO-[N-ALPHA-ACETYL-L-THI ALYSYL-O-PHOSPHOTYROSYL-VALYL-ASPARAGYL-VALYL-PROLYL] (PKF273-791)

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
PROTEIN (PKF273-791) Chain: L
Molecule details ›
Chain: L
Length: 7 amino acids
Theoretical weight: 843 Da
Source organism: Synthetic construct
Expression system: Not provided
Growth factor receptor-bound protein 2 Chain: A
Molecule details ›
Chain: A
Length: 117 amino acids
Theoretical weight: 13.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62993 (Residues: 49-163; Coverage: 53%)
  • Best match: P62993-2 (Residues: 60-122)
Gene names: ASH, GRB2
Sequence domains: SH2 domain
Structure domains: SHC Adaptor Protein

Ligands and Environments

No bound ligands

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM1A
Spacegroup: P321
Unit cell:
a: 83.78Å b: 83.78Å c: 31.99Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.197 0.197 0.232
Expression systems:
  • Not provided
  • Escherichia coli