PDBe 1bjp

X-ray diffraction
2.4Å resolution

CRYSTAL STRUCTURE OF 4-OXALOCROTONATE TAUTOMERASE INACTIVATED BY 2-OXO-3-PENTYNOATE AT 2.4 ANGSTROMS RESOLUTION

Released:

Function and Biology Details

Reaction catalysed:
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate = (3E)-2-oxohex-3-enedioate. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-hydroxymuconate tautomerase Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 62 amino acids
Theoretical weight: 6.82 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: Q01468 (Residues: 2-63; Coverage: 98%)
Gene name: xylH
Sequence domains: Tautomerase enzyme
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: R32
Unit cell:
a: 78.7Å b: 78.7Å c: 314.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.206 0.244
Expression system: Escherichia coli