PDBe 1bjo

X-ray diffraction
2.8Å resolution

THE STRUCTURE OF PHOSPHOSERINE AMINOTRANSFERASE FROM E. COLI IN COMPLEX WITH ALPHA-METHYL-L-GLUTAMATE

Released:

Function and Biology Details

Reaction catalysed:
(1) O-phospho-L-serine + 2-oxoglutarate = 3-phosphonooxypyruvate + L-glutamate. (2) 4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4- phosphonooxybutanoate + L-glutamate. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Phosphoserine aminotransferase Chain: A
Molecule details ›
Chain: A
Length: 360 amino acids
Theoretical weight: 39.71 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P23721 (Residues: 3-362; Coverage: 99%)
Gene names: JW0890, b0907, pdxC, pdxF, serC
Sequence domains: Aminotransferase class-V
Structure domains:
Phosphoserine aminotransferase Chain: B
Molecule details ›
Chain: B
Length: 360 amino acids
Theoretical weight: 39.94 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P23721 (Residues: 3-362; Coverage: 99%)
Gene names: JW0890, b0907, pdxC, pdxF, serC
Sequence domains: Aminotransferase class-V
Structure domains:

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ELLIOTT GX-20
Spacegroup: P212121
Unit cell:
a: 68.86Å b: 94.45Å c: 131.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.185 0.251
Expression system: Not provided